Liu Z, Zheng H, Gottschalk M, Bai X, Lan R, Ji S, et al. Serotypes and putative virulence markers of Streptococcus suis isolates from cats and dogs. Streptococcus suis infections in humans: the Chinese experience and the situation in North America. Streptococci and enterococci associated with tonsils of cattle. 841–55.Ĭruz Colque JI, Devriese LA, Haesebrouck F. Ames, Iowa: Wiley-Blackwell Publishing (2012). 10th ed In: Zimmerman J, Karriker J, Ramirez L, Schwartz A, Stevenson K, editors. MALDI-TOF MS PCR Streptococcus suis biochemical tests identification.
These data indicate an excellent performance of MALDI-TOF MS for the identification of S. suis isolates (96.9%) were correctly identified using the updated database. According to this cut-off value, a total of 125 S. Considering the results of the present study, we suggest using a less restrictive threshold score of ≥2.000 for reliable species identification of S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7, and 9, most isolates had statistically significant higher score values (mean score: 2.65) than those obtained using the original database (mean score: 2.182). After updating the currently available S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard.
4 Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.3 Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain.2 Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.1 Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain Campus de Excelencia Internacional (CEI) Moncloa, Universidad Politécnica de Madrid (UPM), Universidad Complutense de Madrid (UCM), Madrid, Spain.
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